Neutral–Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures

Title: Neutral–Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures
Creator: Mettrick, Karla A.; Weaver, Georgia M.; Grainge, Ian
Relation: Methods in Molecular Biology 2119
Publisher Link: http://dx.doi.org/10.1007/978-1-0716-0323-9_5
Publisher: Humana
Resource Type: book chapter
Date: 2020
Description: Neutral–neutral 2-dimensional agarose gel electrophoresis enables the detection of replication intermediate structures in DNA. Here we describe how DNA from Escherichia coli cells can be purified to retain replication intermediates and then be separated by size and shape using two consecutive agarose gel electrophoresis protocols. The DNA structures present within a localized region can be visualized by a Southern blotting/radioactive hybridisation protocol.
Subject: DNA replication; 2-dimensional agarose gel; replication intermediates; Southern blotting
Identifier: http://hdl.handle.net/1959.13/1439705
Identifier: uon:41005
Identifier: ISBN:9781071603222
Language: eng